Differences in the growth of Candida albicans fungi on alternative media of soybean (Glycine max (L.) Merrill) and Sabouraud Dextrose Agar (SDA)

Fina Fitrotul Azlina; Eka Puspitasari; Qurrotu A'yunin Lathifah; Imelda Putri K.

doi:10.30872/jsel.v1i1.6520

Authors

Fina Fitrotul Azlina STIKES Hutama Abdi Husada
Eka Puspitasari STIKES Hutama Abdi Husada
Qurrotu A'yunin Lathifah STIKES Hutama Abdi Husada
Imelda Putri K. STIKES Hutama Abdi Husada

DOI:

https://doi.org/10.30872/jsel.v1i1.6520

Keywords:

Alternative medium, Candida albicans, Sabouraud Dextrose Agar, Soybean medium

Abstract

Candida albicans is an opportunistic pathogenic fungus that can cause candidiasis. However, culture media commonly used for its growth, such as Sabouraud Dextrose Agar (SDA), are relatively expensive and not always readily available. Soybeans (Glycine max (L.) Merrill) contain various nutrients, including carbohydrates, proteins, fats, vitamins, and minerals, which may support fungal growth and serve as an alternative culture medium. This study aimed to determine the difference in the growth of Candida albicans on an alternative soybean medium and SDA. A quantitative experimental approach with a Post-Test Only Control Group Design was employed. The study used Candida albicans ATCC 14053 isolates, which were inoculated onto both the soybean alternative medium and SDA and incubated for 24 hours. Fungal growth was evaluated by counting the number of colonies formed on each medium. The results showed that the mean number of Candida albicans colonies on the soybean medium was 98 colonies, whereas the mean number on SDA was 31 colonies. Statistical analysis using the Independent Samples t-test revealed a significant difference in fungal growth between the two media (p = 0.002; α = 0.05). These findings indicate that the soybean alternative medium supports the growth of Candida albicans significantly better than SDA and has potential as a cost-effective and environmentally friendly alternative culture medium for fungal cultivation.

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