Aktivitas Antibakteri Sabun Organik Berbasis Ekstrak Kopi Robusta (Coffea canephora) Terhadap Escherichia coli ATCC 8739

Fatimah Fatimah; Maya Istyadji; Yudha Irhasyuarna Fatimah

doi:10.30872/jibs.v12i1.6592

Authors

Fatimah Fatimah Lambung Mangkurat University
Maya Istyadji
Yudha Irhasyuarna Fatimah

DOI:

https://doi.org/10.30872/jibs.v12i1.6592

Keywords:

Antibacterial Activity, Escherichia coli, Organic Soap, Robusta Coffee

Abstract

The purpose of this study was to determine the effectiveness of robusta coffee extract-based organic soap (Coffea canephora) against the growth of Escherichia coli ATCC 8739 bacteria. This study used a completely randomized design (CRD) with seven treatment groups of robusta coffee soap at concentrations of 10%, 25%, 50%, 75%, 100%, 150%, and 300%, a positive control (ampicillin), and a negative control (aquadest). The disc diffusion method was used to evaluate antibacterial activity. Data were analyzed using one-way ANOVA and Tukey's post-hoc test. The results showed that the inhibition zone diameter increased with increasing concentration of robusta coffee extract against E. coli. At concentrations ≥150%, the inhibition zone was equivalent to or greater than the positive control ampicillin, with significant differences (p < 0.05). These results suggest that robusta coffee soap has potential as an environmentally friendly natural antibacterial alternative and can promote the use of local robusta coffee.

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