Comparison of cell disruption methods for improving lipid extraction from Porphyridium cruentum
Abstract
Cell disruption and extraction processes are very important downstream processing steps in microalgae-based in industrial bioprocesses. Each type of microalgae has a varied cell wall structure, so it is necessary to optimize an effective and economical cell disruption method to maximize the extraction process. This study aimed to evaluate several cell disruption methods, including: osmotic shock method (NaCl 20% w/v), acid method (HCl 3M), and microwave (450W, 5 min) for lipid extraction for Porphyridium cruentum biomass. Lipid extraction was carried out using n-hexane as solvent. Among several tested methods, the highest yield of lipid extraction was obtained by using acid method (8.15 ± 0.12% w/w), while the microwave method only produced lipids of 2.79 ± 0.37% w/w, and the lowest yield was obtained by using osmotic shock (1.07 ± 0.44 % w/w). The acid method is a very simple method for disruption of P. cruentum cells, because it does not involve the use of expensive equipment. Therefore, this method is considered efficient and economical for use in the extraction process of lipid or other biomolecule compounds.
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Byreddy, A.R., Gupta, A., Barrow, C.J., & Puri, M. (2015). Comparison of cell disrution methods for improving lipid extraction from Thraustochytrid Strains. Mar. Drugs, 13(8), 5116. https://doi.org/10.3390/md13085111.
Chisti, Y. (2007). Biodiesel from microalgae. Biotech adv., 25(3), 294-306. https://doi.org/10.1016/j.biotechadv.2007.02.001.
Gujar, A., Cui, H., Ji, C., Kubar, S., & Li, R. (2019). Development, production and market value of microalgae products. Applied Microbiology Open Access, 5(2), 162. https://doi.org/10.35248/2471-9315.19.5. 162.
Lee, J.-Y., Yoo, C., Jun,S.-Y., Ahn, C.-Y., & Oh, H.-M. (2010). Comparison of several methods for effective lipid extraction from microalgae. Bioresour. Technol., 101(1), 75-77. https://doi.org/10.1016/j.biortech.2009.03.058.
Laurens, L.M.L., Nagle, N., Davis, S., Sweeney, N., van Wychen, S., Lowell, A., & Pienkos, P.T. (2015). Acid-catalyzed algal biomass pretreatment for integrated lipid and carbohydrate-based biofuels production. Green Chem., 17(2), 1145–1158. https://doi.org/10.1039/C4GC01612B.
Magota, A., Saga, K., Atobe, S., & Imou, K. (2012). Effect of thermal pretreatments on hydrocarbon recovery from Botryococcus braunii. Bioresource Technology, 123, 195-198. https://doi.org/10.1016/j.biortech.2012.07.095.
Purwanti, A. (2014). Pengambilan lipid dari mikroalga basah dengan cara ekstraksi dalam autoklaf. ST AKPRIND Yogyakarta.
Rakesh, S., Dhar, D.W., Prasanna, R., Saxena, A.K., Saha, S., Shikla, M., & Sharma, K. (2015). Cell disruption methods for improving lipid extraction efficiency in unicellular microalgae. Eng. Life Sci., 14(4), 1–5. https://doi.org/10.1002/elsc.201400222.
Ramola, B., Kumar, V., Nanada, M., Mishra, Y., Tyagi, T., Gupta, A., & Sharma, N. (2019). Evaluation, comparison of different solvent extraction, cell disruption methods and hydrothermal liquefaction of Oedogonium macroalgae for biofuel production. Biotechnol Rep (Amst) (22), e00340. https://doi.org/10.1016/j.btre.2019.e00340.
Foo, S.C., Yusoff, F, Md., Ismail, M., Basri, M., Chan, K.W., Khong, N.M.H., & Yau, S.K. (2015). Production of fucoxanthin-rich fraction (FxRF) from a diatom, Chaetoceros calcitrans (Paulsen) Takano 1968. Algal Research, 12, 26-32. https://doi.org/10.1016/j.algal.2015.08.004.
Setyaningsih, I., Salamah, E., & Rahman, D.A. (2013). Komposisi kimia dan aktivitas antihiperglikemik biomassa dan polisakarida ekstraseluler dari mikroalga Porphyridium cruentum. Jurnal Pengolahan Hasil Perikanan Indonesia, 16(1), 80. https://doi.org/10.17844/jphpi.v16i1.7777.
Singh, N.K. & Dhar, D.W. (2011). Microalgae as second generation biofuel. A review. Agron Sustain Develop, 31(4), 605–629. https://doi.org/10.1007/s13593-011-0018-0.
Wang, D., Li, Y., Hu, X., Su, W., & Zhong, M. (2015). Combined enzymatic and mechanical cell disruption and lipid extraction of green alga Neochloris oleoabundans. Int. J. Mol. Sci, 16(4), 7707-7722. https://doi.org/10.3390/ijms16047707.
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