Aktivitas antioksidan ekstrak etanol daging buah rambutan hitam (Nephelium ramboutan-ake) dengan metode DPPH, ABTS dan FRAP

Antioxidant activity of rambutan hitam (Nephelium ramboutan-ake) flesh ethanol extract utilizing DPPH, ABTS, and FRAP methods

Authors

  • Lizma Febrina Fakultas Farmasi Universitas Mulawarman
  • Rolan Rusli Fakultas Farmasi, Universitas Mulawarman
  • Erwin Samsul Fakultas Farmasi, Universitas Mulawarman
  • Arman Rusman Fakultas Farmasi, Universitas Mulawarman
  • Widiastuti Agustina Eko Setyowati Jurusan Pendidikan Kimia, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Sebelas Maret

DOI:

https://doi.org/10.30872/bcsj.v8i1.4839

Keywords:

ABTS, Antioksidan, DPPH, FRAP, Maritam

Abstract

Rambutan hitam atau maritam (Nephelium ramboutan-ake) merupakan tumbuhan tropis khas Kalimantan Timur. Tumbuhan ini tumbuh liar dihutan-hutan Kalimantan. Secara empiris, masyarakat setempat menggunakan buah rambutan hitam untuk pengobatan kanker. Namun demikian, penelitian ilmiah mengenai rambutan hitam masih sangat terbatas. Oleh karenanya, penelitian ini bertujuan untuk menganalisis aktivitas antioksidan dari buah rambutan hitam dengan menggunakan metode DPPH, ABTS, dan FRAP. Analisis aktivitas antioksidan dilakukan secara kolorimetri menggunakan spektrofotometer UV-Vis pada panjang gelombang tertentu. Hasil penelitian ini mengungkapkan bahwa ekstrak etanol daging buah rambutan hitam memiliki aktivitas antioksidan dengan kategori kuat baik dianalisis menggunakan metode DPPH (IC50 = 32,51 ± 0,21 mg/L), ABTS (IC50 = 30,04 ± 0,31 mg/L), dan FRAP (EC50 = 34,22 ± 0,17 mg/L). Aktivitas antioksidan yang kuat dari rambutan hitam menggambarkan potensi yang besar dari rambutan hitam dalam mengurangi dampak negatif radikal bebas. Adapun aktivitas antioksidan buah rambutan hitam dilaporkan pertama kali pada tulisan ini.

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References

Adaku, C., Skaar, I., Gumisiriza, H., Byamukama, R., Jordheim, M., & Andersen, O. M. (2020). Anthocyanin profile and antioxidant activity of edible leaves of Dissotis brazzae Cogn (Melastomataceae). Journal of Food and Nutrition Sciences, 8(6), 139-145. https://dx.doi.org/10.11648/j.jfns.20200806.11

Ayoka, T. O., Ezema, B. O., Eze, C.N., & Nnadi, C. O. (2022). Antioxidants for the prevention and treatment of non-communicable diseases. Journal of Exploratory Research in Pharmacology, 7(3), 179–189. https://dx.doi.org/10.14218/jerp.2022.00028

Basu, A., Izuora, K., Betts, N. M., Ebersole, J. L., & Scofield, R. H. (2021). Dietary strawberries improve biomarkers of antioxidant status and endothelial function in adults with cardiometabolic risks in a randomized controlled crossover trial. Antioxidants, 10(11), 1-8. https://dx.doi.org/10.3390/antiox10111730

Belayneh, A., Chelkeba, L., Amare, F., Fisseha, H., Abdissa, S. G., Kaba, M., Patel, S. A., & Ali, M. K. (2024). Investigation of non-communicable diseases prevalence, patterns, and patient outcomes in hospitalized populations: a prospective observational study in three tertiary hospitals. Journal of Health, Population and Nutrition, 43(128), 1-11. https://dx.doi.org/10.1186/s41043-024-00599-z

Benzie, I. F. F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Analytical Biochemistry, 239(1), 70–76. https://doi.org/10.1006/abio.1996.0292

Chan, G. K., Goh, B. H., Kamarudin, M. N. A., & Kadir, H. A. (2012). Aqueous fraction of Nephelium ramboutan-ake rind induces mitochondrial-mediated apoptosis in HT-29 human colorectal adenocarcinoma. Molecules, 17(6), 6633–6657. https://doi.org/10.3390/molecules17066633

Crisóstomo, L., Oliveira, P. F., Alves, M. G. (2022). Antioxidants, oxidative stress, and non-communicable diseases. Antioxidants, 11(6), 1080. https://doi.org/10.3390/antiox11061080

Febrina, L., Happyana, N., & Syah, Y. M. (2021). Metabolite profiles and antidiabetic activity of the green beans of Luwak (civet) coffees. Food Chemistry, 355, 1-9. https://doi.org/10.1016/j.foodchem.2021.129496

Febrina, L., Happyana, N., & Syah, Y. M. (2023). Metabolic profiling, antioxidant activity, and alpha-glucosidase inhibitory activity of the roasted beans of Luwak (civet) coffee. ACS Food Science and Technology, 3(11),1864–1876. https://doi.org/10.1021/acsfoodscitech.3c00249

Flieger, J., Flieger, W., Baj, J., & Maciejewski, R. (2021). Antioxidants: classification, natural sources, activity/capacity measurements, and usefulness for the synthesis of nanoparticles. Materials, 14(15), 1-54. https://doi.org/10.3390/ma14154135

Floegel, Kim, D.O., Chung, S., Koo, S.I., & Chun, O.K. (2011). Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. Journal of Food Composition and Analysis, 24(7), 1043–1048. https://dx.doi.org/10.1016/j.jfca.2011.01.008

George, J., Edward, D., Pun S., & Williams, D. (2022). Evaluation of antioxidant capacity (ABTS and CUPRAC) and total phenolic content (Folin-Ciocalteu) assays of selected fruit, vegetables, and spices. International Journal of Food Science, 1(1), 1-18. https://dx.doi.org/10.1155/2022/2581470

Gupta, M., Dey, S., Marbaniang, D., Pal, P., Ray, S., Mazumder, B. (2020). Grape seed extract: having a potential health benefits. Journal of Food Science and Technology, 57(4), 1205–1215. https://dx.doi.org/10.1007/s13197-019-04113-w

Martin, M. S., Goncalves, A. C., Alves, G., & Silva, L. R. (2023). Blackberries and mulberries: Berries with significant health-promoting properties. International Journal of Molecular Science, 24(15), 1-34. https://doi.org/10.3390/ijms241512024

Ozgen, M., Reese, R. N., Tulio, A. Z., Scheerens, J. C., Miller, A> R. (2006). Modified 2,2-Azino-Bis-3-Ethylbenzothiazoline-6-Sulfonic Acid (ABTS) method to measure antioxidant capacity of selected small fruits and comparison to ferric reducing antioxidant power (FRAP) and 2,2′-Diphenyl-1- Picrylhydrazyl (DPPH) methods. Journal of Agricultural and Food Chemistry, 54(4), 1151–1157. https://dx.doi.org/10.1021/jf051960d

Panova, I. G., & Tatikolov, A. S. (2023). Endogenous and exogenous antioxidants as agents preventing the negative effects of contrast media (contrast-induced nephropathy). Pharmaceuticals, 16(8), 1-27. https://dx.doi.org/10.1007/s13197-019-04113-w

Putri, A.F. (2021). Mengenal Buah Eksotik Kalimantan. Yayasan Fastabiqul Khairot

Putri., A. S., Pasedan, W. F., Kusuma, I. W., & Kuspradini, H. (2021). Antioxidant and antibacterial activity from three different solvents of Nephelium ramboutan-ake leaves crude extract. Proceedings of the Joint Symposium on Tropical Studies (JSTS-19), 11, 14–17. https://dx.doi.org/10.2991/absr.k.210408.003.

Rahaman, M. M., Hossain, R., Herrera-Bravo, J., Islam, M. T., Atolani, O., Adeyemi, O. S., Owolodun, O. A., Kambizi, L., Daştan, S. D., Calina, D., Sharifi-Rad, J. (2023). Natural antioxidants from some fruits, seeds, foods, natural products, and associated health benefits: An update. Food Science and Nutrition, 11(4), 1657-1670. https://dx.doi.org/10.1002/fsn3.3217

Sham, N., Qin, C., Zhu, Z., Redington, C. G., Xiao, H., Bai, Q., Wakefield, M. R., Kou, L., Fang, Y. (2021). Raspberry extract with potential antitumor activity against cervical cancer. Anticancer Research, 41(7), 3343-3348. https://doi.org/10.21873/anticanres.15122

Shehata, M. G., Abu-Srie, M. M., El-Aziz, N. M. A., & El‑Sohaimy, S. A. (2021). Nutritional, phytochemical and in-vitro anticancer potential of sugar apple (Annona squamosa) fruits. Scientific Repots, 11(6224), 1-13. https://doi.org/10.1038/s41598-021-85772-8

Shi, N., & Chen, T. (2022). Chemopreventive properties of black raspberries and strawberries in esophageal cancer. Antioxidants, 11(9), 1-14. https://doi.org/10.3390/antiox11091815

Shi, L., Zhao, W., Yang, Z., Subbiah, V., & Suleria, H. A. R. (2022). Extraction and characterization of phenolic compounds and their potential antioxidant activities. Environmental Science and Pollution Research, 29, 81112–81129. https://doi.org/10.1007/s11356-022-23337-6

Stefano, V. D., Buzzanca, C., Melilli, M. G., Indelicato, S., Mauro, M., Vazzana, M., Arizza, V. Lucarini, M., Durazzo, A., & Bongiorno, D. (2022). Polyphenol characterization and antioxidant activity of grape seeds and skins from sicily: A preliminary study. Sustainability, 14(11), 2-10. http://dx.doi.org/10.3390/su14116702

Zhang, J., Gao, N., Shu, C., , Cheng, S., Sun, X., Liu, C., Sin, G., Li, B., & Tian, J. (2021). Phenolics profile and antioxidant activity analysis of kiwi berry (Actinidia arguta) flesh and peel extracts from four regions in China. Frontiers in Plant Science, 12, 1-13. https://dx.doi.org/10.3389/fpls.2021.689038

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Published

2025-08-06

How to Cite

Febrina, L., Rusli, R., Samsul, E., Rusman, A., & Setyowati, W. A. E. (2025). Aktivitas antioksidan ekstrak etanol daging buah rambutan hitam (Nephelium ramboutan-ake) dengan metode DPPH, ABTS dan FRAP: Antioxidant activity of rambutan hitam (Nephelium ramboutan-ake) flesh ethanol extract utilizing DPPH, ABTS, and FRAP methods . Bivalen: Chemical Studies Journal, 8(1), 11–19. https://doi.org/10.30872/bcsj.v8i1.4839

Issue

Vol. 8 No. 1 (2025)

Section

Articles

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Copyright (c) 2025 Lizma Febrina, Rolan Rusli, Erwin Samsul, Arman Rusman, Widiastuti Agustina Eko Setyowati

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